Opti Science GFPIII General Fluorescence Probe

Application • Detection of GFP, green fluorescent protein • Detection of most other fluorescent proteins • Detection of specialized fluorescent compounds down to the ppb range • Transgenic crop research & development • Detect genetically modified organisms • Gene expression studies • Detection & measurement of contaminants • Pesticide / Chemical spray analysis • Parts inspection Features Lightweight, Hand-Held Design Optimized for Field Work • Discrete sample measurement - solid, plant, animal tissue or liquid • Single sampling or continuous monitoring rates from1 per second to 1 per minute. • User Changeable source & detect filter modules • Bright Color Graphic data display • 8 Hrs use on two AA rechargeable batteries • Data logging up for up to 2 gigabytes of data • Stand Alone Operation- No P.C. Required The GFPIII is a general purpose modulated fiber optic probe fluorometer designed for a variety of measurement applications. It is available with a choice of excitation and detection modules to meet most fluorescence measurement requirements including popular selections such as; Chlorophyll, Rhodamine, Fluorescein, GFP, and most other Fluorescent protein compounds. Custom modules can also be created for special applications, with filters available from the UV to the near infrared range. As a hand-held, field portable instrument, the light weight and easy to carry GFPIII incorporates on-board data logging and a bright TFT color graphics touch sensitive display screen for system parameters and data display. Up to two gigabytes of data, traces, calibration tables, and user settings can be stored on the instrument. Rechargeable AA batteries will commonly allow the instrument to run for up to eight hours before recharging. A second set of batteries are included with the instrument. A metal leaf clip is provided for those doing transgenic and GFP detection studies as well. Opti-Sciences has designed this instrument to provide the most reliable results available by including temperature compensation and multi-point calibration capabilities over the measuring range selected. References for GFP Meter ( all versions) . Li1 J., Brunner A.M., Meilan R., Strauss S.H. ( 2009) Stability of transgenes in trees: expression of two reporter genes in poplar over three field seasonsTree Physiol ( 2009) 29 ( 2) : 299-312 Li1 J., Brunner A.M., Meilan R., Strauss S.H.1, ( 2008) Matrix attachment region elements have small and variable effects on transgene expression and stability in field-grown PopulusArticle first published online: 7 SEP 2008 DOI: 10.1111/ j.1467- 7652.2008.00369.x Plant Biotechnology Journal Volume 6, Issue 9, pages 887896, December 2008 Stuart M., Dignan C., McClary D., Golder Associates ( NZ) Ltd, Dunedin, NZ. Ltd, Takapuna, NZ. ( 2008) Evaluation of marine response tools: Subtidal containment and treatment system MAF Biosecurity New Zealand Technical Paper No: 2010/ 20 Prepared for MAF Biosecurity New Zealand By ISBN 978-0-478-37081-2 ( print) ISBN 978-0-478- 37082-9 ( online) ISSN 1179-5832 ( print) ISSN 1179-5840 ( online) December 2008 Millwood R.J., Moon H. S., and Stewart C. N., ( 2008) Fluorescent Proteins in Transgenic Plants Reviews in Fluorescence 2008, Reviews in Fluorescence, Volume 2008, 387-403, DOI: 10.1007/ 978-1-4419-1260-2_ 16 Leckie B. M. and Stewart C.N., ( 2008) Agroinfiltration as a technique for rapid assays for evaluating candidate insect resistance transgenes in plants From the issue entitled " Special Issue: Plant biotechnology in support of the Millennium Goals ( Volume I) " Plant Cell Reports Volume 30, Number 3, 325-334, DOI: 10.1007/ s00299-010-0961-2 Palumbo RE., and Veilleux R.E., ( 2007) Variation of GFP expression in diploid and tetraploid transgenic potato American Journal of Potato Research Volume 84, Number 5, 393-401, DOI: 10.1007/ BF02987185 Matthew D. Halfhill R., Millwood R.J, Stewart C.N., ( 2004) Green Fluorescent Protein Quantification in Whole Plants , Reginald J. and C. Neal Transgenic Plants: Methods and Protocols Methods in Molecular Biology, 2004, Volume 286, IV, 215-225, DOI: 10.1385/ 1-59259-827-7: 215 Millwood R.J. , Halfhill1 M.D. , Harkins D. Russotti R., and Stewart, Jr. C.N. ( 2003) Instrumentation and Methodology for Quantifying GFP Fluorescence in Intact Plant Organs, UNC Greensboro, Greensboro, NC, 1NC State University, Raleigh, NC, and Opti-Sciences, Tyngsboro, MA, USA BioTechniques 34: 638-643 ( March 2003) Halfhill M.D., Millwood R.J. , Rufty T.W. , Weissinger A.K. and Stewart C.N. ( 2004) Spatial and temporal patterns of green fluorescent protein ( GFP) fluorescence during leaf canopy development in transgenic oilseed rape, Brassica napus L. Plant Cell Reports Volume 22, Number 5, 338-343, DOI: 10.1007/ s00299-003-0696-4